TABLE 1.
Bacterial strains and plasmids used in this studya
| Strain or plasmid | Relevant characteristics | Source |
|---|---|---|
| X. oryzae pv. oryzicola | ||
| RS105 | Wild type, causal agent of bacterial leaf streak in rice, Rifr | This lab |
| RΔhrpG | hrpG deletion mutant of RS105, Rifr | 2 |
| RΔhrpX | hrpX deletion mutant of RS105, Rifr | 2 |
| RΔhrpV | hrcV deletion mutant of RS105, Rifr | 2 |
| RΔhrcT | hrcT deletion mutant of RS105, Rifr | 2 |
| RΔBP | RS105 mutant containing deletion of hrpB1 promoter and insertion of Gmr, Rifr | This study |
| C1RΔhrcT | Complemented strain of RΔhrcT with plasmid pChrcT1, Rifr Gmr | This study |
| C2RΔhrcT | Complemented strain of RΔhrcT with plasmid pChrcT3, Rifr Gmr | This study |
| C1RΔhrcTXac | Complemented strain of RΔhrcT with plasmid pC1HrcTXac, Rifr Gmr | This study |
| C1RΔhrcTRs | Complemented strain of RΔhrcT with plasmid pC1HrcTRs, Rifr Gmr | This study |
| C1RΔhrcTPst | Complemented strain of RΔhrcT with plasmid pC1HrcTPst, Rifr Gmr | This study |
| C2RΔhrcTXac | Complemented strain of RΔhrcT with plasmid pC2HrcTXac, Rifr Gmr | This study |
| C2RΔhrcTRs | Complemented strain of RΔhrcT with plasmid pC2HrcTRs, Rifr Gmr | This study |
| C2RΔhrcTPst | Complemented strain of RΔhrcT with plasmid pC2HrcTPst, Rifr Gmr | This study |
| X. axonopodis pv. citri | Strain 306 | Collected by this lab |
| R. solanacearum | Strain GMI1000 | Collected by this lab |
| P. syringae pv. tomato | Strain DC3000 | Collected by this lab |
| E. coli | ||
| DH5α | F− ϕ80lacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) phoA supE44λ− thi-1 gyrA96 relA1 | Clontech |
| BL21(DE3) | F− ompT hsdS20 gal | Novagen |
| Plasmids | ||
| pMD18-T | pUC ori, cloning vector, Apr | TaKaRa |
| pUFR034 | incW mob(p) mob+ lacZA+, PK2 replicon, Kmr | 36 |
| pBBR1MCS-5 | Broad-host-range cloning vector, Gmr | 49 |
| pKMS1 | Suicide vector derived from pK18mobGII, sacB+, Kmr | This lab |
| pT1GUS | pUFR034 expressing gusA under 350-bp promoter region of hrpB operon, Kmr | 2 |
| pT3GUS | pUFR034 expressing gusA under 500-bp promoter region of hrcT, Kmr | This study |
| pXGUS | pUFR034 expressing gusA under 300-bp promoter region of hrpX, Kmr | This study |
| pGGUS | pUFR034 expressing gusA under 490-bp promoter region of hrpG, Kmr | This lab |
| pKMSΔGm | 1,977-bp fragment containing the left and right border fragments of the hrpB operon promoter, flanks the Gm ORF in pKMS1, Kmr | This study |
| pChrcT1 | pBBR1MCS-5 expressing X. oryzae pv. oryzicola hrcT from a 350-bp promoter region derived from hrpB operon, Gmr | This study |
| pChrcT3 | pBBR1MCS-5 expressing hrcT from a 500-bp promoter region of hrcT Gmr | This study |
| pC1HrcTXac | pBBR1MCS-5 expressing hrcT from X. axonopodis pv. citri 306; promoter was derived from 350-bp promoter region derived from hrpB operon, Gmr | This study |
| pC1HrcTRs | pBBR1MCS-5 expressing hrcT from R. solanacearum GMI1000; promoter was derived from 350-bp promoter region derived from hrpB operon, Gmr | This study |
| pC1HrcTPs | pBBR1MCS-5 expressing hrcT from P. syringae pv. tomato DC3000; promoter was derived from 350-bp promoter region derived from hrpB operon, Gmr | This study |
| pC2HrcTXac | pBBR1MCS-5 expressing hrcT from X. axonopodis pv. citri 306; promoter was derived from 500-bp region of X. oryzae pv. oryzicola hrcT, Gmr | This study |
| pC2HrcTRs | pBBR1MCS-5 expressing hrcT from R. solanacearum GMI1000; promoter was derived from 500-bp region of X. oryzae pv. oryzicola hrcT, Gmr | This study |
| pC2HrcTPst | pBBR1MCS-5 expressing hrcT from P. syringae pv. tomato DC3000; promoter was derived from 500-bp region of hrcT, Gmr | This study |
| pHZWavrXa27 | AvrXa27 with Flag tag under the control of lacZ promoter, Spr, Apr | Yang Bing's lab |
| pET30a(+) | pBR322 origin, lacI, His tag/S tag; Kmr | Novagen |
| pETHrcTΔ41N | hrcT from RS105 cloned in pET30a(+) as a 711-bp fragment, His tag at C terminus, Kmr | This study |
| phrpX | The 300-bp promoter of hrpX fused to a promoterless gusA in pBI121, Kmr | This study |
| HrcT | hrcT from RS105 fused under CaMV 35S promoter in pCAMBIA1300, Kmr | This study |
| pOs8N3 | Os8N3 promoter fused to a promoterless gusA in pBI121, Kmr | 37 |
| PthXo1 | pthXo1 fused under CaMV 35S promoter in pCAMBIA1300, Kmr | 37 |
a
Apr, ampicillin resistance; Kmr, kanamycin resistance; Rifr, rifampin resistance; Spr, spectinomycin resistance; Gmr, gentamicin resistance.