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GM1-ELISA measurement for determination of functional activity of LTB protein expressed in the outer membrane of S. Enteritidis-LTB ghost. Outer membrane preparations prepared from S. Enteritidis-LTB ghost or S. Enteritidis ghost was added to the wells coated with GM1 protein and then anti-LTB antiserum was added to identify ganglioside-bound LTB. A higher OD value was obtained from wells coated containing outer membrane preparation of S. Enteritidis-LTB ghost.
