TABLE 1.

Proteins of vaccinia virus recognized by antibody from vaccinia virus-naive or Dryvax- and ACAM2000-vaccinated individuals

Protein	Descriptiona	Location of viral antigenb	Nonvaccinated prevalence (%)	Vaccinated prevalence (%)	P value	References
F13L	IEV membrane wrapping palmytilated protein	EEV membrane	2	99	3.86E−39	52–55
A10L	Core protein P4a, assembly of nucleoprotein complex	IMV core	3	97	1.67E−35	54–58
D13L	Rifampin resistance protein, spicule coat formation of IV	IMV membrane	2	93	2.61E−33	54, 56, 59, 60
D8L	Cell surface chondroitin sulfate binding protein	IMV membrane	2	69	2.67E−19	54–56, 58, 61
C3L	Complement regulatory protein	EC/EEV membrane	8	79	4.93E−19	48, 62, 63
A13L	IV to IMV assembly phosphoprotein	IMV membrane	6	77	8.13E−19	54–56, 58, 64
A33R	EEV glycoprotein, actin tail formation with A36R	EEV membrane	3	68	9.88E−18	55, 65, 66
A11R	IMV membrane assembly protein	IC, viral factory	13	84	1.39E−16	54, 67
I1L	Intermediate-class gene promoter	IMV core	3	62	4.08E−15	54–56, 68
H3L	IMV heparan sulfate binding surface protein, IMV assembly	IMV membrane	10	70	5.57E−14	54–56, 58, 69, 70
A27L	Heparan sulfate binding surface protein, IMV microtubule-dependent transport	IMV membrane	32	92	1.32E−12	54–56, 58, 71, 72
C21/B27R	Ankyrin-like protein	Unknown	11	77	1.61E−11
B5R	Plaque size, host range protein precursor, EEV formation and dissemination	EEV membrane	11	63	4.27E−10	55, 73–75
A38L	Integrin/CD47-associated protein, Ca2+ influx	Host cell membrane	2	40	2.26E−08	76, 77
G5R	Putative FEN1-like nuclease	IMV corec	10	45	1.33E−06	78, 79
A26L	IMV A-type inclusion, laminin binding protein	IMV membrane	14	60	1.56E−06	54, 56, 80
I3L	Single-stranded DNA-binding phosphoprotein	IMV core	6	34	2.21E−05	54–56, 81, 82
J6R	DNA-directed RNA polymerase 147-kDa subunit	IMV core	3	40	2.28E−05	54–56, 83, 84
L4R	Single- and double-stranded DNA and ssRNA-binding virion core protein vp8	IMV core	10	41	3.95E−05	54–56, 58, 85, 86
O1L	ERK1/2 signaling modulator	ICd	5	33	4.37E−05	49
B19R	IFN-α/β receptor	EC, host cell membrane	2	25	9.65E−05	51, 87, 88

^aIEV, intracellular enveloped virion; IV, immature virion; IMV, intracellular mature virus; EEV, extracellular enveloped virus; ssRNA, single-stranded RNA.

^bEEV, extracellular enveloped virus; IMV, intracellular mature virus; EC, extracellular; IC, intracellular.

^cIt should be noted that while da Fonseca et al. (78) found G5R to be located within the IMV, other studies (54–56) did not.

^dThe study of Manes et al. (89) in 2008 suggests that O1L may also be detected within VACV virion particles.