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Acquisition of gene cassettes. Integrons acquire new gene cassettes by recombination between the attC of a circular cassette and the attI site of the integron. This inserts incoming cassettes at a position proximal to the integrase gene and its embedded promoter. Cassette arrays can expand by repeated cassette acquisition, but cassettes can also be excised as closed circles by attI × attC or attC × attC recombination.
