FIG 1.

TcBDF3 is a cytoplasmic bromodomain-containing protein in epimastigotes. (A) Nuclear (N) and nonnuclear (NN) protein extracts (30 μg per well) were subjected to Western blot analysis using rabbit anti-TcBDF3 antibodies, anti-TcTAT (cytosolic), anti-TcHMGB (nuclear), anti-TcBDF2 (nuclear), and anti-T. cruzi H4 histone (TcH4) (nuclear). On the left is the Coomassie-stained gel. (B) Enzymatic activity of αHAdH and MdH in epimastigotes treated with increasing concentrations of digitonin. Activities were measured and normalized to the protein concentration in the extracts. The arrows below the graph indicate the digitonin concentrations at which cytosolic (C), glycosomal (G), and mitochondrial (M) proteins are released. (C) Equal volumes of selected soluble (S) and insoluble (P) fractions obtained at different digitonin amounts (0 to 0.7 mg) were subjected to Western blot analysis using anti-TcBDF3 antibodies (TcBDF3) and known markers for different organelles. The antibodies used were anti-TcTAT (cytosolic), anti-TcMdHglyc (glycosomal), anti-TcMdHmit (mitochondrial), anti-TcPAR2 (flagellar), and anti-α-tubulin (α-tubulin) (cytoskeletal). TL, total lysate.