TcBDF3 is localized at the cytoplasm, the flagellum, and the flagellar pocket of epimastigotes. (A to C and E) Immunoelectron microscopy of TcBDF3 in T. cruzi epimastigotes using purified rabbit anti-TcBDF3 antibodies. The nucleus (N), kinetoplast (K), flagellar pocket (FP), and flagellum (F) are indicated. Gold particles are indicated with black and white arrows. The white arrows indicate flagellar labeling. (D) Enlarged image of the boxed area in panel C. (F) Enlarged image of the boxed area in panel E. Bars = 1 μm.