FIG 3.

TcBDF3 is expressed throughout the T. cruzi life cycle in vitro. (A) Western blot analysis using purified rabbit anti-TcBDF3 antibodies (a-TcBDF3) and mouse anti-α-tubulin (a-α-tubulin) as a loading control. A, amastigote total protein extracts; E, epimastigote total protein extracts; T, trypomastigote total protein extracts (30 μg per well). (B) Immunofluorescence assay using purified anti-TcBDF3 and parasites at different stages of the T. cruzi life cycle. A, free amastigote; Ac, amastigotes inside a Vero cell; E, epimastigote; T, trypomastigote from infected Vero cells; Tm, metacyclic trypomastigote obtained in vitro. Anti-rabbit IgG conjugated to fluorescein was used as a secondary antibody. Nuclei and kinetoplasts were labeled with DAPI. Bars = 2 μm.