FIG 4.

TcBDF3 changes its location during in vitro metacyclogenesis. Immunofluorescence assays used purified rabbit anti-TcBDF3 (α-TcBDF3) and mouse monoclonal anti-acetylated α-tubulin (α-AcTub) antibodies in intermediate stages 1a, 1b, and 1c, as defined by Ferreira et al. (51). On the right are schematic diagrams of the positions of the flagellum (F), nucleus (N), and kinetoplast (K) in the three selected intermediate differentiation stages. Anti-rabbit IgG conjugated to fluorescein (green) and anti-mouse IgG conjugated to rhodamine (red) were used as secondary antibodies. Nuclei and kinetoplasts were labeled with DAPI (blue).