FIG 5.

TcBDF3 is detected in the cytoskeletons and flagella of epimastigotes (E) and only in the flagella of metacyclic trypomastigotes (T). Immunofluorescence assays used purified rabbit anti-TcBDF3 (α-TcBDF3) and mouse monoclonal anti-acetylated α-tubulin (α-AcTub) (A) and mouse anti-PAR2 (α-PFR) (B) antibodies on isolated cytoskeletons and flagella of epimastigotes and metacyclic trypomastigotes. Anti-rabbit IgG conjugated to fluorescein (green) and anti-mouse IgG conjugated to rhodamine (red) were used as secondary antibodies. The last right lanes are enlarged images of the detergent-resistant structures that correspond to MTs attached to basal bodies and forming the flagellar pocket. The green arrowheads indicate TcBDF3 localization, and the red arrowheads indicate acetylated α-tubulin (A) and PAR2 (B) localization in these structures.