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. 2014 Jun;13(6):776–784. doi: 10.1128/EC.00041-14

TABLE 1.

Identified interactors

Protein identification no.a Name and/or description Mol wt No. of peptides Interactorb Reference(s) S. salmonicida GenBank no.c
Axonemal/cytoskeleton
    GL50803_111950 Axonemal dynein heavy chain 570,319 900 Precedents 32, 39 EST41976, 0.0
    GL50803_101138 Axonemal dynein heavy chain 578,219 849 Precedents 32, 39 EST46166.1, 0.0
    GL50803_40496 Axonemal dynein heavy chain 553,424 778 Precedents 32, 39 EST44588.1, 0.0
    GL50803_13273* P28 axonemal dynein light chain 26,895 294 IP+++ 32, 39 EST43975.1, 6E–138
    GL50803_42285 Axonemal dynein heavy chain 11 834,759 48 Precedents 32, 39 EST41750.1, 2E–61
    GL50803_6744* Centrin 18,687 46 IP+ 32, 39 EST41812.1, 2E–98
    GL50803_37985 Dynein heavy chain 118,679 26 Precedents 32, 39 EST48250.1, 0.0
    GL50803_137716 Axoneme-associated protein GASP-180 174,782 24 No
    GL50803_16424* Myeloid leukemia factor like 29,702 14 IP− 35 EST47319.1, 9E–17
    GL50803_14242 Dynein heavy chain-cytoplasmic 633,335 11 EST46283.1, 1E–27
Chaperone
    GL50803_11992 TCP-1 chaperonin subunit epsilon 61,193 223 Precedents 29 EST46493.1, 0.0
    GL50803_11397 TCP-1 chaperonin subunit beta 56,604 204 Precedents 29 EST41446.1, 0.0
    GL50803_16124 TCP-1 chaperonin subunit eta 64,752 196 Precedents 29 EST48572.1, 0.0
    GL50803_13500 TCP-1 chaperonin subunit theta 60,646 187 Precedents 29 EST46649.1, 0.0
    GL50803_10231 TCP-1 chaperonin subunit zeta 60,941 118 Precedents 29 EST48976.1, 0.0
    GL50803_17482 TCP-1 chaperonin subunit delta 56,324 86 Precedents 29 EST41630.1, 4E–173
    GL50803_91919 TCP-1 chaperonin subunit alpha 59,281 82 Precedents 29 EST47029.1, 0.0
    GL50803_17411 TCP-1 chaperonin subunit gamma 61,559 71 Precedents 29 EST41533.1, 0.0
    GL50803_88765* Cytosolic HSP70 71,633 22 IP+ 52 EST45839, 0.0
    GL50803_17121 Bip 74,360 12 53 EST46254.1, 0.0
Nuclear
    GL50803_9825* TBP-interacting protein TIP49 51,418 276 IP+++ 54–56 EST49365.1, 0.0
    GL50803_17565 TBP-interacting protein TIP49 52,616 162 Precedents 54–56 EST48144.1, 0.0
    GL50803_15113* ARP7-like 51,465 90 IP+ 54–56 EST44310.1, 6E–31
    GL50803_9705 Hypothetical/YEATS domain 30,449 23 EST47502.1, 1E–27
    GL50803_8125 SMARCC1 47,004 17 Precedents 54–56 No
    GL50803_2851 Histone acetyltransferase MYST2 49,916 12 Precedents 54–56 EST45122.1, 9E–78
    GL50803_6886 Prokaryotic SMC domain protein 102,836 11 No
    GL50803_17461 SWIRM domain protein 124,241 7 EST47570.1, 3E–51
Signaling
    GL50803_6317 Putative DUB 150,389 16 EST43894.1,1E–14
    GL50803_22850* ERK7-like/giERK2 41,096 15 IP+ EST48827.1, 0.0
    GL50803_6430* 14-3-3 protein 28,576 8 IP++ EST46224.1, 6E–115
    GL50803_12795 Phosducin-like 26,919 6 EST41453.1, 1E–14
    GL50803_9413 Protein disulfide isomerase PDI2 50,408 6 Precedents 57 EST43183.1, 5E–43
Trafficking
    GL50803_14373* Dynamin 79,513 13 IP− 36 EST46023.1, 0.0
Unknown/Giardia specific
    GL50803_15264 Hypothetical protein 404,245 153 No
    GL50803_39938 Hypothetical protein 716,474 95 EST41505.1, 4E–25
    GL50803_17532 Hypothetical protein 66,334 57 EST45241.1, 3E–19
    GL50803_15485 Hypothetical protein 751,987 55 EST41949.1, 9E–20
    GL50803_13942 Hypothetical Protein 56,506 53 No
    GL50803_7807 WD-40 repeat protein 59,785 51 EST44362.1, 2E–65
    GL50803_17266 Ankyrin and WD repeat protein 14,462 47 No
    GL50803_137711 Hypothetical protein 719,629 41 EST41949.1, 2E–49
    GL50803_17596 Zinc finger domain protein 90,538 29 EST4938.1, 2E–09
    GL50803_23897 Hypothetical protein 92,642 28 No
    GL50803_99726 Hypothetical protein 11,183 25 No
    GL50803_113592 Hypothetical protein 489,165 24 No
    GL50803_5859 Hypothetical Protein 20,141 22 No
    GL50803_20601 Hypothetical protein 11,136 22 No
    GL50803_3559 Hypothetical protein 24,801 18 No
    GL50803_14492 Hypothetical protein 54,020 15 No
    GL50803_35487 Hypothetical protein 884,517 14 No
    GL50803_8725 Hypothetical protein 51,753 11 No
    GL50803_37350 Hypothetical protein 796,898 11 EST43354.1, 0.0
    GL50803_35341 Hypothetical protein 776,894 11 EST45092.1, 1E–176
    GL50803_15442 KIF binding domain 108,189 8 No
    GL50803_137739 Hypothetical protein 211,474 8 No
    GL50803_92602 Hypothetical protein 342,328 6 EST44629.1, 6E–73
a

*, This protein was chosen for testing interaction with actin, as described in Results.

b

+++, strong interactor; ++, moderate interactor; +, weak interactor; −, no interaction detected.

c

The exponential values are BLAST Expect values, indicating the level of conservation between the homologs.