FIG 6.

Ptp2 may have Hog1-independent targets. (A) Each strain (WT [strain H99] and hog1, ptp2, PTP2oe, and PTP2oe hog1 mutant strains) was grown overnight at 30°C in liquid YPD medium, 10-fold serially diluted, and spotted onto YPD medium containing fludioxonil (1.5 μg/ml), fluconazole (14 μg/ml), diamide (3 mM), SDS (0.04%), tunicamycin (0.3 μg/ml), hydroxyurea (110 mM), CdSO₄ (27.5 μM), H₂O₂ (3.5 mM), and flucytosine (600 μg/ml). Cells were incubated at 30°C for 2 to 4 days and photographed. (B) Effects of PTP2 overexpression on capsule production. The relative packed cell volume was measured by calculating the ratio of the length of packed cell volume phase to the length of the total volume phase. *, P < 0.05; NS, not significant (P > 0.05). (C) Each indicated set of α cells were cocultured with KN99a on V8 medium in the dark at room temperature and photographed after 10 days.