FIG 8.

Hog1 and Ptp1 play opposing roles in sexual differentiation and adherence/invasion. (A) Each indicated α strain (WT [strain H99] and hog1, ptp1, and PTP1oe hog1 mutant strains) was cocultured with KN99a on V8 medium in the dark at room temperature and photographed after 13 days. (B) Northern blot analysis for monitoring pheromone gene (MFα1) expression was performed with total RNA isolated from solo cultures or cocultures of the following strains, which were grown for 24 h under mating conditions: WT (strain H99) and hog1 (YSB64), PTP1oe (YSB2586), PTP1oe ptp2 (YSB2572), PTP1oe hog1 (YSB2655), PTP2oe (YSB2569), PTP2oe ptp1 (YSB2590), PTP2oe hog1 (YSB2658) mutants. (C) Each strain was grown in liquid YPD medium for 16 h at 30°C. Five microliters of the culture was spotted onto filament agar plates and incubated at room temperature for 14 days. Agar adherence was determined by washing the plate under gently flowing water for 20 s. Agar invasion was assessed by gently rubbing each colony with a gloved finger under a water stream. After washing, colonies were photographed.