FIG 9.

Cellular localization of Ptp1 and Ptp2 and their Hog1-anchoring function. (A) Strains expressing Ptp1-GFP (YSB2785) or Ptp2-GFP (YSB2891) were cultured in YPD liquid medium at 30°C for 16 h and subcultured in YPD liquid medium containing 1 M NaCl for the indicated times. (B) Roles of Ptp1 and Ptp2 in the cellular localization of Hog1. Hog1-GFP expressing strains, in which PTP1 or PTP2 was deleted (YSB2558 and YSB2563, respectively), were exposed to 1 M NaCl for the indicated times as described for panel A. The cellular localization of each GFP fusion protein was visualized by fluorescence microscopy. For DAPI staining of the nucleus, cells were fixed by formaldehyde. Bars, 10 μm.