TABLE 1.
C. neoformans strains used in this study
| Strain | Relevant genotypea | Parent | Reference |
|---|---|---|---|
| H99 | MATα | 51 | |
| KN99a | MATa | 52 | |
| YSB42 | MATα cac1Δ::NAT-STM#159 | H99 | 25 |
| YSB119 | MATα aca1Δ::NAT-STM#43 ura5 ACA1-URA5 | YSB108 | 25 |
| YSB121 | MATa aca1Δ::NEO ura5 ACA1-URA5 | YSB108 | 25 |
| YSB64 | MATα hog1Δ::NAT-STM#177 | H99 | 13 |
| YSB242 | MATα hog1Δ::NAT-STM#177 ura5 URA5-PACT1:HOG1-FLAG-GFP | YSB114 | 13 |
| YSB253 | MATα hog1Δ::NAT-STM#177 HOG1T171A+Y173A-NEO | YSB64 | 13 |
| YSB308 | MATα hog1Δ::NAT-STM#177 HOG1K49S+K50N-NEO | YSB64 | 13 |
| YSB676 | MATα atf1Δ::NAT-STM#220 | H99 | 32 |
| YSB275 | MATα ptp2Δ::NAT-STM#184 | H99 | This study |
| YSB1704 | MATα ptp1Δ::NAT-STM#125 | H99 | This study |
| YSB1492 | MATa ptp2Δ::NEO | KN99a | This study |
| YSB1619 | MATa ptp1Δ::NAT-STM#125 | KN99a | This study |
| YSB2058 | MATα ptp1Δ::NAT-STM#125 ptp2Δ::NEO | YSB1704 | This study |
| YSB2635 | MATα ptp1Δ::NAT-STM#125 PTP1-NEO | YSB1704 | This study |
| YSB2195 | MATα ptp2Δ::NAT-STM#184 PTP2-NEO | YSB275 | This study |
| YSB1617 | MATα hog1Δ::NAT-STM#177 ptp2Δ::NEO | YSB64 | This study |
| YSB2586 | MATα PH3:PTP1-NEO | H99 | This study |
| YSB2572 | MATα PH3:PTP1-NEO ptp2Δ::NAT-STM#184 | YSB275 | This study |
| YSB2655 | MATα PH3:PTP1-NEO hog1Δ::NAT-STM#177 | YSB64 | This study |
| YSB2569 | MATα PH3:PTP2-NEO | H99 | This study |
| YSB2590 | MATα PH3:PTP2-NEO ptp1Δ::NAT-STM#125 | YSB1704 | This study |
| YSB2658 | MATα PH3:PTP2-NEO hog1Δ::NAT-STM#177 | YSB64 | This study |
| YSB2558 | MATα ptp1Δ::NEO | YSB242 | This study |
| YSB2563 | MATα ptp2Δ::NEO | YSB242 | This study |
| YSB2785 | MATα PTP1-GFP-NEO | YSB1704 | This study |
| YSB2891 | MATα PTP2-GFP-NEO | H99 | This study |
| YSB2563 | MATα hog1Δ::NAT-STM#177 ura5 URA5-PACT1:HOG1-FLAG-GFP ptp2Δ::NEO | YSB242 | This study |
| YSB2558 | MATα hog1Δ::NAT-STM#177 ura5 URA5-PACT1:HOG1-FLAG-GFP ptp1Δ::NEO | YSB242 | This study |
a
NAT-STM#, Natr marker with a unique signature tag. PACT1 and PH3 indicate ACT1 and H3 promoters, respectively.