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Single gmt mutations have modest effects on protein glycosylation. Total protein fractions of the strains indicated were resolved by SDS-PAGE and immmunoblotted with antibody specific for terminal α-1,6-mannose residues or mannoproteins, as indicated below. Panel A, 10% gel; panel B, 12% gel. *, examples of bands that migrate faster in gmt1 cells; the wild type was loaded twice to facilitate comparison. For each panel, the same amount of protein was loaded in all lanes.
