FIG 9.

Gmt proteins are located in the Golgi complex. Chromosomally expressed Gmt1-HA and Gmt2-Flag were immunolocalized and imaged as detailed in the text. Shown are single-channel and merged images of signal derived from staining with DAPI (4′,6-diamidino-2-phenylindole) (nucleus) and with antibodies to the two tags as indicated. Arrowheads indicate examples of regions where Gmt1 and Gmt2 do not colocalize; broken white lines indicate the cell edge as observed under bright-field microscopy. Scale bar, 5 μm.