FIG 2.

Insertions of active RNA Pol II-transcribed genes can abrogate AGC. Strains were grown in either SC-leucine (A and B), SC media (C and D), or YPD (E and F), subjected to a 37°C heat shock, and monitored for their expression profiles at the EBP2, MPP10, and YJR003C genes by RT-PCR. The profiles of the leftward-oriented MPP10.LEU2 insert (YMM554) are represented in panels A and C, and the profiles of the rightward-oriented MPP10.LEU2 insert (YMM559) are presented in panels B and D. (E) YMM555 MPP10. tRNA (Thr); (F) YMMM556 MPP10.Ty tRNA (Thr).