TABLE 2.
Oligonucleotides used in this study
| Name | Forward primer (5′–3′) | Annealing site | Reverse primer (5′–3′) | Annealing site | Use |
|---|---|---|---|---|---|
| ACT1qRT | ATCGTTATGTCCGGTGGTACC | 1196 | TGGAAGATGGAGCCAAAGC | 1281 | qPCR |
| KANqRT | CACCGGATTCAGTCGTCACTCATGG | 559 | GGCAAGATCCTGGTATCGGT CTGCGATTCC | 684 | qPCR |
| EBP2qRT | AACGCTACCTTACAGAAACG | 957 | TCCGTTAGGCCTGCCTCTATCGAA | 1122 | qPCR |
| MPP10qRT | CGAGGAGGAGGAGGCTATTTAT | 674 | CTTCCTCATCCGCAAATAAGTC | 844 | qPCR |
| YJR003CqRT | ACCACCATTGACCCATACTCTC | 147 | GACCACTTCCATCAGTTCATCA | 447 | qPCR |
| SAG1-NSA-1 | GGTTACTTTGAGCACACGGCTTTG | −634 | GATACCGGACAATTGGCTTCCTG | −475 | NSA |
| SAG1-NSA-2 | CTCATCTCAGGGAACGAAAATTG | −566 | CATAGGTTGAAATCATGAGAGAAG | −32 | NSA |
| SAG1-NSA-3 | CTCAGCTGAGCTCGGTTCGATC | −410 | GAAGAAAAAAAGAGCCAGGATG | −272 | NSA |
| YJR003C-NSA-1 | GTACAGTTAGTACATTGAGTC | −346 | GACGGAAAAGGATAAGAACTAG | −205 | NSA |
| YJR003C-NSA-2 | CATCCTGGCTCTTTTTTTCTTC | −294 | CTATGATAAAATCTGCGGTG | −182 | NSA |
| YJR003C-NSA-3 | CTAGTTCTTATCCTTTTCCGTC | −224 | GAAACAGCCTTCGGGTAATG | −105 | NSA |
| YJR003C-NSA-5 | CATTACCCGAAGGCTGTTTC | −124 | GAATGGCGGTTAGCTGTTAAG | −5 | NSA |
| YJR003C-NSA-6 | CACCATGAAAGAGTTCGATGAG | −64 | GAGAGAGTAAAACCTCTTGTTAG | 57 | NSA |
| YJR003C-NSA-7 | CTTAACAGCTAACCGCCATTC | −26 | GCTTTTTGATTATGTTCTTTC | 92 | NSA |
| YJR003C-NSA-8 | CATTCTGCGGCTACGTTATCTAAC | 15 | GATGACGAATTGGATCGAAAG | 129 | NSA |
| YJR003C-NSA-9 | ATAGAAAGAACATAATCAAAAAGC | 68 | CATTCTGCGATAGAGAGTATG | 181 | NSA |
| YJR003C-NSA-10 | CTTTCGATCCAATTCGTCATC | 108 | CATATTGTGTACCATGGCCGCATC | 243 | NSA |
| YJR003C-NSA-11 | CATACTCTCTATCGCAGAATG | 160 | CAACGCCGTAGTCAAGATCAC | 280 | NSA |
| YJR003C-NSA-12 | GATGCGGCCATGGTACACAATATG | 219 | GTTTGATGGCGGGAAGTGAAG | 343 | NSA |
| YJR003C-NSA-13 | GTGATCTTGACTACGGCGTTG | 259 | CAAGAGCTTTGTACTCTTCCTG | 383 | NSA |
| MPP10-NSA-1 | TTATGACTATCATTCCTATCGCAAAG | −640 | GAAGGCCTTTCGCAGCTCTTC | −506 | NSA |
| MPP10-NSA-2 | GTATTGGACGTTCTGATGAATGTG | −584 | GCTATCAAAACGAAGACAAC | −460 | NSA |
| MPP10-NSA-3 | GAAGAGCTGCGAAAGGCCTTC | −526 | GTAAAACACAAACCGGCCCCCAG | −400 | NSA |
| MPP10-NSA-4 | GTTGTCTTCGTTTTGATAGC | −479 | GTTACGTGACAAGCCACTCTCTC | −313 | NSA |
| MPP10-NSA-5 | CTGGGGGCCGGTTTGTGTTTTAC | −422 | CACCAACACCTAATGTGGACAAC | −272 | NSA |
| MPP10-NSA-6 | GAGAGAGTGGCTTGTCACGTAAC | −335 | CAGAAGTACAGAGCTAATG | −197 | NSA |
| MPP10-NSA-7 | GTTGTCCACATTAGGTGTTGGTG | −294 | GAAAAGGCGGTGAATATTTTATG | −124 | NSA |
| MPP10-NSA-8 | CATTAGCTCTGTACTTCTG | −215 | CGTGCACACATCATTTATTCATAAC | −24 | NSA |
| MPP10-NSA-9 | CGTAATATACATATTTTCGTGTAG | −179 | GACATTATACGACTTTCCTTGGGTC | 5 | NSA |
| MPP10-NSA-10 | CTGGCCGCCGGCATGCGAG | −119 | CAATACTCCAAAGAGTTCTGAC | 24 | NSA |
| MPP10-NSA-10.5 | GAAACAGTGTTTTGTTATGAATAAATG | −61 | GCTTTAACATCTTTAGAAGTGGC | 86 | NSA |
| MPP10-NSA-11 | GTGCACGAAAGACCCAAGGAAAG | −30 | CTTACAGGTATTGATAACTGAATC | 117 | NSA |
| MPP10-NSA-12 | GTCAGAACTCTTTGGAGTATTG | 3 | CATCAACAGTGATTTCGTCCAG | 166 | NSA |
| MPP10-NSA-13 | GATTCAGTTATCAATACCTGTAAG | 94 | GATCACCATCAATACTATCAAGAA | 229 | NSA |
| MPP10-NSA-14 | CTGGACGAAATCACTGTTGATG | 145 | GGCGTGACAACATCTTTGAGTTCTTG | 269 | NSA |
| MPP10-NSA-15 | TTCTTGATAGTATTGATGGTGATC | 206 | CTTTCACTTGATCTCCTCCGTC | 340 | NSA |
| HSP104-NSA-1 | GAAACCGTGGATGTTCAGGAC | −700 | CGCCTTTGAATCGATGACAAT | −588 | NSA |
| HSP104-NSA-2 | CACCGAGCCGGGGAAATTCG | −657 | GTCGTCGATCCAGTCCATTTC | −546 | NSA |
| HSP104-NSA-3 | ATTGTCATCGATTCAAAGGCG | −608 | GCCCTTGGAGTTTGGATTCTTG | −501 | NSA |
| HSP104-NSA-5 | CAAGAATCCAAACTCCAAGGGC | −522 | GAATAAATAAGTGAATAGGTAG | −413 | NSA |
| HSP104-NSA-6 | GGTTTAAAAACCTTCTGCACCA | −474 | CGATGGAGGGTTCAATGTTAAT | −358 | NSA |
| HSP104-NSA-7 | CTACCTATTCACTTATTTATTC | −434 | TAACCCTTCTAGAAAATTCTGG | −279 | NSA |
| HSP104-NSA-8 | ATTAACATTGAACCCTCCATCG | −379 | CTTTGAGATGGGCCCCCTGTTG | −203 | NSA |
| HSP104-NSA-9 | CCAGAATTTTCTAGAAGGGTTA | −300 | GTTTGCGCCCCTTTGCCTTTTAC | −161 | NSA |
| HSP104-NSA-11 | GTAAAAGGCAAAGGGGCGCAAAC | −183 | TTGCTGATTCGATTCAAGGG | −52 | NSA |
| HSP104-NSA-12 | GGCATTGTAATCTTGCCTCAATTCC | −132 | CTGTATATTTTATGGTACGTGTAG | −5 | NSA |
| HSP104-NSA-13 | CCCTTGAATCGAATCAGCAA | −71 | GCCAACGTCAAAATCGTTAGAGCCC | 53 | NSA |
| HSP104-NSA-14 | CTACACGTACCATAAAATATACAG | −29 | GTATAGGTTGTAATTGTGGATG | 100 | NSA |
| HSP104-NSA-15 | GGGCTCTAACGATTTTGACGTTGGC | 29 | CTGATCCATCTTCTGGCGTTTC | 142 | NSA |
| HSP104-NSA-16 | CATCCACAATTACAACCTATAC | 79 | GATCATAGTCGTAACGGCCC | 190 | NSA |
| HSP104-NSA-17 | GAAACGCCAGAAGATGGATCAG | 121 | GCAGGTTGCTGTTGAGGAATTC | 245 | NSA |
| HSP104-NSA-18 | GGGCCGTTACGACTATGATC | 171 | CCCCAAAGCATAACTTGGAG | 279 | NSA |
| HSP104-NSA-19 | GAATTCCTCAACAGCAACCTGC | 224 | GCGCTATAAATGAGTCCTTCTG | 337 | NSA |