FIG 3.

Proliferation of smTc3 and smPTP cells is not affected by heterologous dsRNA expression. The smTc3 (A) and smPTP (B) cell lines are single-marker BF cell lines in which stem-loop constructs were integrated into the RRNA spacer for inducible expression of Tc3 and PTP dsRNA, respectively. (Insets) Addition of doxycycline to the medium did not inhibit cell proliferation in either cell line while it induced strong expression of Tc3 or PTP RNA, as analyzed by semiquantitative PCR of random-hexamer-derived cDNA. n.i., noninduced; 1, 1 day induced.