FIG 5.

Defining the locations of the nuclear localization signals of BORG. The full-length BORG RNA is shown on top, with the progressively truncated mutants of Exon2 (a) and Exon3 (b) shown below. The thin lines connect regions of BORG RNA that are included in each construct. The numbers indicate the beginning and end of the included sequences relative to the 5′ end of full-length BORG. A(n) denotes the location of the poly(A) tail of each RNA. The arrows show the position of the AGCCC motif(s) in each exon, with the numbers next to the arrows denoting the position of each motif from the 5′ end of full-length BORG. The cellular localization of each transcript is determined by RT-PCR assays using radiolabeled primers and shown to the right of the corresponding construct. Nuc and Cyt refer to nuclear and cytoplasmic fractions, respectively.