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. 2014 Jun;34(12):2294–2307. doi: 10.1128/MCB.00388-14

FIG 3.

FIG 3

Activities of point mutants of eEF2K. (A) Activities of WT and D284N GST-eEF2K against the MH-1 peptide. Data are means ± SEM (n = 3). ***, P < 0.001; ****, P < 0.0001. (Inset) Levels of autophosphorylation of WT and D284N. (B) Activities of WT and D284N eEF2K against eEF2. Assays were performed within the linear range. Samples were analyzed by SDS-PAGE and phosphorimaging. The gel was also stained with Coomassie brilliant blue to confirm equal amounts of eEF2. (C) Activities of selected point mutants against MH-1. All assays were performed within the linear range of the assay. Data are means ± SEM (n = 3). **, P < 0.01; ****, P < 0.0001. P values were obtained using a two-way ANOVA compared with the WT followed by Bonferroni post hoc tests. (D) Activities of WT (lane 1) and D280A (lane 4) forms of eEF2K were determined against eEF2. All assays were performed within the linear range of the assay. Samples were analyzed by SDS-PAGE and autoradiography. The gel was also stained with Coomassie brilliant blue to confirm equal amounts of eEF2.