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. 2014 Jun;34(12):2264–2282. doi: 10.1128/MCB.01542-13

FIG 9.

FIG 9

PEA15 inhibits RAS-induced transformation and is downregulated in multiple cancer types. (A) Immunoblot analysis of PEA15 protein from MEF/SV40-ER cells transduced with an empty or HRAS v12 expression vector. Actin expression is probed as a loading control. (B) Soft-agar assay of MEF/SV40-ER cells transduced with vectors expressing the indicated genes or treated with 10 μM U0126. Magnified images (40×) from representative wells are shown. (C) Tumorigenesis assay (n = 5) of MEF/SV40-ER cells expressing HRAS v12 and transduced with the indicated vectors. Tumor volumes calculated at the indicated days after injections are shown. (D) Immunoblot analysis of PEA15 levels in MEL-ST cells transduced with control (empty) or HRAS v12 expression vectors. Actin was used as a loading control. (E) Soft-agar proliferation assay of MEL-ST cells transduced with control, PEA15, HRAS v12, or both PEA15 and HRAS v12 expression vectors. Representative images are shown.