FIG 2.

SRSF10 is required for hormone-induced adipogenesis in different cell models. (A) WT and SRSF10 KO primary MEF cells were treated with insulin, IBMX, and dexamethasone plus troglitazone for induction of adipogenesis. At day 9 postinduction, adipocyte differentiation was assessed by ORO staining. (B) WT and KO primary MEF cells were induced to differentiate as described for panel A. RNAs were extracted from these cells at indicated induction days. Relative PPARγ or AdipoQ mRNA levels were measured by real-time PCR and normalized to 36b4 mRNA. Values shown are the mean ± SD (n = 3). The significance of each detected change was evaluated by Student's t test. (C) C3H10T1/2 cells were infected with retroviruses expressing either SRSF10-specific shRNA (shSRSF10) or control shRNA (shCtrl) and then selected for puromycin resistance. Western blotting was carried out to confirm SRSF10 knockdown efficiency in selected cells. Antitubulin blotting was used as a loading control. (D) shSRSF10 and shCtrl C3H10T1/2 stable cells were induced to differentiate as for panel A. Cells were stained with ORO at day 6 postinduction. (E) RNA was extracted from shSRSF10 or shCtrl C3H10T1/2 stable cells at the indicated days and the relative level of PPARγ or AdipoQ mRNA was measured as for panel B.