FIG 2.

The conserved Eya tyrosine phosphatase domain is ubiquitinated. (A and B) The C terminus of Eya1 is ubiquitinated. Flag-tagged Eya1 truncated fragments (as indicated) and HA/UB were coexpressed in HEK293 cells, and total cell lysates were subjected to an IP/IB assay at 48 h after transfection. Results are summarized in panel B. Lane 1, total cell lysate; lane 2, IP with control IgG; lane 3, IP with a Flag-specific antibody. (C) Ubiquitination patterns of several Flag-tagged Eya1 variants that were found in human branchio-oto-renal (BOR) syndrome. WT, wild type.