FIG 5.

(A and B) Prooncogenic activity of Sp1 in pancreatic cancer cells. Cell growth (A) and cell cycle progression (B). Panc1 and L3.6pL cells were transfected with two different oligonucleotides that silence Sp1 (siSp1I and siSp1II), and cell proliferation and cell cycle progression were determined by cell counting or FACS analysis as outlined in Materials and Methods. (C and D) Pancreatic cancer cell invasion and quantitation (C) and induction of apoptosis (D). Panc1 and L3.6pL cells were transfected with siSpI or siSp1II, and inhibition of cell migration in a Boyden chamber assay and induction of apoptosis were determined and quantitated relative to the control (siCT) as outlined in Materials and Methods. Results in panels A to C are expressed as means ± SE for at least 3 replicate determinations for each treatment group, and significant (P < 0.05) increases (*) or decreases (**) compared to a control group (siCT) are indicated.