FIG 7.

Dimer/oligomer formation by α-helix domain mutants of agnoprotein. (A) MBP and MBP-Agno were expressed in E. coli, purified, and analyzed by SDS-8% PAGE, followed by Coomassie blue staining, as described in Materials and Methods. (B to D) Alanine (Ala, A) substitution mutations (I30A, L33A, I30+L33A, E34A, E34+D38A, L29A, L32A, L36A, L32A+L36A, L29A+L32A+L36A) were expressed in E. coli, purified, and analyzed by SDS-8% PAGE, followed by Coomassie blue staining, as described in Materials and Methods. Arrows, a distinct band migrating faster than the WT monomer; bracket (in panel D), degraded agnoprotein monomers; lanes Markers and M, molecular size markers (in kilodaltons).