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(A) Expression of EGFP-S1 fusion proteins in BHK-21 cells; (B) Western blot of expressed EGFP-S1 fusion proteins using rabbit anti-EGFP antibody (left) and SARS-convalescent human serum (right). The molecular masses are given on the right. BJ, LY, Rp3, and E, respectively, represent EGFP-S1 proteins of SARS CoV BJ01, bat SARS-like CoVs LYRa11 and Rp3, and EGFP control.
