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. 2014 Jun;88(12):6983–6992. doi: 10.1128/JVI.00620-14

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FIG 3 — Agilent Bioanalyzer 2100 analyses (with a DNA 7500 chip) of PCR products. (Upper panels) Gel view of Agilent traces. Bands representing dup⁺ and dup⁻ genomes are marked by arrows labeled accordingly. (Lower panels) Bar plots of relative quantities of PCR products determined by Agilent software, representing dup⁺ and dup⁻ genomes. (A) Analysis of recent BVDV-2c strains. NRW 19-13-8a (lane 7) depicts results for the NRW 19-13-8 isolate, reisolated from the animal trial. (B) Analysis of BVDV-2a 890 wild-type strain (v890WT) and clones. Results obtained for plasmids of the variants with (p890FL dup⁺) and without (p890FL dup⁻) duplication and the p890FL dup⁺-derived in vitro transcript are shown. In addition, results for virus rescued from in vitro-transcribed RNA and passaged in cell culture 2 (v890FL dup⁺ P2), 3 (v890FL dup⁺ P3), and 4 (v890FL dup⁺ P4) times are displayed.