FIG 4.

Analysis of replication ability and ADV resistance of HBV mutants derived from clinical isolates harboring the rtA181T, rtV191I, and rtI233V mutations. (A) Sequence analysis of clones harboring the rtA181T, rtV191I, and rtI233V mutations. The substitutions in the overlapping surface protein and the relative replication levels compared to WT are indicated. (B and C) Ten selected clones were transfected into Huh7 cells, and the replication ability and ADV susceptibility were analyzed by Southern blotting. ADV treatment (20 μM) lasted for 4 days. Intracellular HBsAg was detected by Western blotting with an anti-HBs antibody (Abcam).