FIG 3.

HCMV inhibition blocks the mislocalization of IFI16. HELFs were infected with wild-type or UV-inactivated HCMV (1 PFU/cell; 1.2 J/cm² for two pulses) and treated with phosphonoformic acid (PFA; 100 μM) or ganciclovir (GCV; 100 μM) as indicated. Cells were fixed 72 h later in 1% paraformaldehyde and processed by immunofluorescence analysis for IFI16 (green) and gB (red) (left panel) or subjected to combined FISH with a BAC DNA probe containing the entire HCMV genome (red) and immunofluorescence analysis with anti-IFI16 antibodies (green); cell nuclei were visualized in blue (right panel). Images were taken by confocal microscopy (×63 magnification).