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. 2014 Jun;88(12):6623–6635. doi: 10.1128/JVI.02765-13

TABLE 1.

Sanger sequencing and deep sequencing of DI and infected AC ferretsa

Protein Nucleotide change (position) Amino acid position Function or domain of mutation (reference[s]) Frequency (%), line A
Expt 2, p10
 Expt 3, p11
DI AC DI AC 1 AC 2
PB2 C(242)T T81Ib NP interaction domain (38) 100 100 100 100 100
PB1 750 G250c 58 86 98
2157 V719c 28 19 100
PA G(204)A P68b,c 98 100 88 99 100
G(243)A G81b,c 99 100 90 96 100
HA G/A(938)G K/R313Rb HA stalk globular head (34, 69) 95 100 46 96 100
G583A G195R 62 40 100 100
NP G(850)A V284Mb PB2 interaction domain (38) 100 100 100 100 100
C(1249)A L417Ib PB2 interaction domain (38) 98
G(546)A V182b,c 100 100 100 100 98
C(1188)T N396b,c 100 100 100 100 100
A(633)G G211b,c 100
NA T(786)A D262Eb Adjacent to NA binding pocket (33) 42 100 35 100
G/A(408)A L136c 99 100 100 100 100
A(972)G V324c 53 99 34 100
A(1071)G G357c 41 100 28 100
M1 G(284)A R95Kb Spherical virion morphology (62) 100 100 100 100 100
G(632)A R211Qb RNP binding domain (63) 100 100 100 100 100
NS1 A(328)G K110Eb Host protein eIF4GI interaction (70) 46 100 56 98
a

Nucleotide and amino acid changes are compared to the published A/ostrich/Italy/2332/2000 (H7N1) sequence that was verified by Sanger sequencing. The changes shown were present at >20% frequency in two or more samples, excluding NP L417I and the silent mutation at NP G211 reported during initial Sanger sequencing of AC p10 line A. Numbering reflects that of the full-length H7N1 amino acid sequence. HA numbering refers to H7 numbering starting at methionine. Absence of a frequency indicates 0% and no changes were found in M2 and NS2. Five mutations present in all DI and AC ferrets are in bold. Mutations also found in A/Anhui/1/2013 and A/Shanghai/2/2013 (H7N9) are underlined.

b

Amino acid and/or nucleotide change found during Sanger sequencing of p10 line A AC.

c

Silent or synonymous mutation.