FIG 2.
Experimental identification of the role of OmpW in VP5 infection. (A) Plaques formed by wild-type strains N16961 and VC631, deletion mutant N16961-dompW, and strains complemented with different plasmids. (B) VP5 adsorption by the wild-type strain N16961 and its ompW mutants. Plaques were counted in 10−5 dilutions in a double-layer agar plate containing the VP5-sensitive strain 2477c. The N16961-dompW mutant was used as the test and calculation control. Three independently prepared VP5 phages were used as the biological repeat tests. The numbers of plaques in the N16961- and N16961-dompW-R-treated groups were divided by the number of plaques in the N16961-dompW-treated group to produce plaque formation percentages to show adsorption ratios. Smaller values indicate that more phage particles were adsorbed. Statistically significant comparisons between the groups are marked with an asterisk (*) (analysis of variance [ANOVA] for randomized block design; P < 0.05). No difference was found between the N16961 and N16961-dompW-R groups.