FIG 6.

CH25H restricts late stages of MHV68 gene expression and viral DNA synthesis in primary macrophages. BL6, CH25H^−/−, or IRF-1^−/− macrophages were infected with MHV68 at an MOI of 5 PFU/cell. (A to E) RNA was isolated at the indicated times postinfection, and the levels of RTA, orf59, and orf40 mRNA were measured by quantitative RT-PCR and subsequently normalized to the corresponding GAPDH mRNA levels. (F) Total DNA was isolated at the indicated times postinfection, and the levels of viral DNA were quantified by real-time PCR and subsequently normalized to the corresponding cellular DNA levels (measured using GAPDH-specific primers). *, P < 0.05.