FIG 1.

Establishment of PEL stable cell lines with KAP1 or Sin3A knockdown. (A) Constitutive knockdown of KAP1 and Sin3A in PEL cells. PEL cell lines BC3 and BCBL1, parental and with KAP1 (shKAP1) or Sin3A (shSin3A) knockdown or scramble control (shCtrl), were individually generated by lentivirus-mediated transduction followed by selection with 2 μg/ml of selection. IB, immunoblotting. (B) Lower KSHV episome copy number in PEL cells with KAP1 or Sin3A constitutive knockdown. The genome DNA extracted from PEL cell lines BC3 and BCBL1 with shKAP1 or shSin3A or scramble control shRNA (shCtrl) from panel A were used to detect the relative intracellular viral episome copy number by quantitative PCR with TR as a target. GAPDH was used as an internal control. The results are presented as the average relative fold compared with parental cells from 3 independent knockdown clones from panel A. Double asterisks indicate a P value less than 0.05.