FIG 2.

Loss of KAP1 or Sin3A dramatically enhances the relative efficiency of KSHV virion induction by TPA-NaB or hypoxia. (A) Equal amounts of parental BC3 cells with constitutive knockdown of KAP1 (shKAP1) or Sin3A (shSin3A) or scramble control (shCtrl) were cultured and exposed to TPA and butyrate (NaB) or 1% oxygen treatment for 0, 18, and 36 h. The supernatant of culture medium was subjected to quantitative PCR with TR or K8 as a target for virion production. The efficiency of extracellular KSHV DNA (virion) induction by TPA-NaB or hypoxia was detected and is presented as relative fold compared with mock treatment before induction. (B) A representative image of human PBMC infection with 36-h hypoxia induction virion from panel A at day 4 postinfection by LANA and DAPI nuclear staining.