FIG 3.

Histology quantitation. (A) Image analysis (strong brown) of F4/80 staining of whole foot sections from WT and CCR2^−/− mice at the indicated times postinfection. The dotted line indicates the upper limit of nonspecific staining in control slides (n = 3 to 5 feet from different mice per time point and mouse strain; 2 sections per foot were analyzed). Statistics were determined by the Kolmogorov-Smirnov test. (B) Image analysis (strong red) of Leder staining of whole foot sections of WT and CCR2^−/− mice at the indicated times postinfection (same numbers of feet as described above for panel A). Statistics were determined by Kolmogorov-Smirnov, Mann-Whitney U, or t tests. (C) Image analysis (strong brown) of ApoTag staining of whole foot sections of WT and CCR2^−/− mice at the indicated times postinfection (same numbers of feet as described above for panel A). Statistics were determined by Kolmogorov-Smirnov tests. (D) Image analysis (strong red) of Chromotrope2R staining of whole foot sections of WT and CCR2^−/− mice at the indicated times postinfection (same numbers of feet as described above for panel A). Statistics were determined by Kolmogorov-Smirnov or Mann-Whitney U tests. (E) WT mice were treated with the CCR2 antagonist MK0815 or diluent (control) after CHIKV infection, and feet were stained with anti-Ly6G (a neutrophil-specific marker), followed by image analysis. Statistics were determined by a Kolmogorov-Smirnov test (n = 4 to 6 feet per group).