FIG 1.

KSHV infection enhances the activity of the HIV-1 LTR. (A) Supernatants from doxycycline- or mock-treated KSHV producer iSLK-219 cells were used to infect NH1 and NH2 cells. As an additional control, NH1 and NH2 cells were treated with supernatants from similarly treated iSLK cells. Luciferase levels were determined at 6 and 12 h postinfection. Western blot analysis of β-tubulin served as a loading control. (B) NH1 and NH2 cells were mock treated or incubated with supernatants from reactivated iSLK-219 cells that were either left untreated or cleared of virus by ultracentrifugation. Luciferase levels were determined at 6 h postinfection. Western blot analysis of β-tubulin served as a loading control. (C) NH1 and NH2 cells were mock treated or incubated with viral supernatants that were heat inactivated or mock treated. Western blot analysis of β-tubulin served as a loading control.