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Effects of KSHV tegument proteins on the HIV-1 LTR. (A and B) NH1 and NH2 cells were transfected with the plasmids indicated. At 48 h posttransfection, cell lysates were prepared and luciferase levels were determined. Expression of transfected ORFs was detected by anti-Strep Western blot analysis. Western blot analysis of β-tubulin served as a loading control. Statistical significance was determined by Student t test (**, P < 0.001; ***, P < 0.0001). MW, molecular weight in thousands.
