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ORF45 mediates activation of the HIV-1 LTR through RSK2. NH1 and NH2 cells were transfected with either WT or DN RSK1, RSK2, or RSK3. At 48 h posttransfection, luciferase levels were determined. Western blot analysis of β-tubulin served as a loading control. Statistical significance was determined by Student t test (***, P < 0.0001).
