FIG 4.

Reverse transcription of a clinical HIV-1 isolate is inhibited by DBR1 depletion and completed in the nucleus. GHOST-R5X4 cells were transfected with D4 or M4 for 48 h and then infected with the HIV-1 clinical clonal isolate ACH 337.40.1c1 and harvested at 2 to 12 h postinfection. The cells were lysed and fractionated, and DNA was isolated to evaluate the synthesis of HIV-1 cDNA by qPCR. The analysis was the same as that described in the legend to Fig. 3. Error bars denote the standard deviations from the means. The data represent those from three independent time course experiments processed in triplicate.