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. 2014 Jun;88(12):7054–7069. doi: 10.1128/JVI.00704-14

FIG 6.

FIG 6

Effects of nuclear entry and DBR1 inhibition on HIV-1 strong-stop cDNA synthesis. HeLa.LPCX cells were transfected with M4, and HeLa.LPCX-HA-CPSF6-358 cells were transfected with D4 or M4 for 48 h, followed by infection with a DNase-treated HIV-1 vector (HR-E). Zero to 24 h later, the cells were either fractionated into nuclear and cytoplasmic fractions utilizing the NP-40 protocol or lysed to prepare whole-cell extracts (Total). DNA was isolated and analyzed by qPCR for the synthesis of strong-stop HIV-1 cDNA, as described in the legend to Fig. 3. (A) M4 treatment of HeLa.LPCX control cells; (B) M4 treatment of HeLa.LPCX-HA-CPSF6-358 cells; (C) D4 treatment of HeLa.LPCX-HA-CPSF6-358 cells. Error bars denote the standard deviations from the means. The data represent those from three independent time course experiments processed in triplicate.