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. 2014 Jun;88(12):7054–7069. doi: 10.1128/JVI.00704-14

FIG 7.

FIG 7

Effects of nuclear entry and DBR1 inhibition on HIV-1 envelope and full-length cDNA synthesis. DNAs from the same cell fractions used for the assays whose results are presented in Fig. 6 were analyzed by qPCR for the synthesis of env and full-length cDNA products, as described in the legend to Fig. 3. (A, C, and E) env reverse transcripts in M4-treated HeLa.LPCX control cells (A), M4-treated HeLa.LPCX-HA-CPSF6-358 cells (C), and D4-treated HeLa.LPCX-HA-CPSF6-358 cells (E); (B, D, and F) full-length cDNAs in M4-treated control cells (B), M4-treated HeLa.LPCX-HA-CPSF6-358 cells (D), and D4-treated HeLa.LPCX-HA-CPSF6-358 cells (F). Error bars denote the standard deviations from the means. The data represent those from three independent time course experiments processed in triplicate.