TABLE 1.

Comparison of RT mutation frequencies versus WT: all classes of mutant lacZα sequences^c

Enzyme ID	Mutation frequencya	Mutation rateb	Raw (FET) P value	BH (FDR)-adjusted P value	BH significance
WT	451/205,171	1.4 × 10−5
Y115F	524/93,725	3.7 × 10−5	<0.0001	<0.0001	****
Q151M	537/209,064	1.7 × 10−5	0.0154	0.0177	*
M184I	479/150,110	2.1 × 10−5	<0.0001	<0.0001	****
M184V	466/172,601	1.8 × 10−5	0.0020	0.0027	**

^aMutation frequency data represent the number of mutant lacZα sequences divided by the total number of recovered clones.

^bMutation rate data represent the mutation frequency divided by the size of the lacZα target sequence. Because the data were obtained with a one-round vector, the rate data were calculated as mutations/bp/generation.

^cRaw (FET) data represent P values of the significance of the results of comparisons to the WT by Fischer's exact test (FET). To control for compounding type I error in these multiple comparisons, the Benjamini-Hochberg (BH) false-discovery-rate (FDR) corrections to significance calculations were applied to the raw (FET) P values where a single asterisk (*) represents 0.05 or less, two asterisks (**) represent 0.01 or less, three asterisks (***) represent 0.001 or less, and four asterisks (****) represent 0.0001 or less. ID, identifier.