TABLE 4.

Comparison of RT mutation frequencies versus WT: class 3 multiple nucleotide substitution mutations (total)^d

Enzyme ID	Mutation frequencya	Mutation rateb	Mutation fractionc (%)	Raw (FET) P value	BH (FDR)-adjusted P value	BH significance
WT	55/205,171	1.8 × 10−6	12
Y115F	42/93,725	2.9 × 10−6	8	0.0155	0.0414	*
Q151M	69/209,064	2.2 × 10−6	13	0.2811	0.3213
M184I	84/150,110	3.7 × 10−6	18	<0.0001	0.0002	***
M184V	68/172,601	2.6 × 10−6	15	0.0371	0.0741

^aMutation frequency data represent the number of mutant lacZα sequences divided by the total number of recovered clones.

^bMutation rate data represent the mutation frequency divided by the size of the lacZα target sequence. Because the data were obtained with a one-round vector, the rate data were calculated as mutations/bp/generation.

^cMutation fraction data represent the number of class-specific mutant lacZα sequences divided by the total number of mutant lacZα sequences.

^dRaw (FET) data represent P values of the significance of the results of comparisons to the WT by Fisher's exact test. To control for compounding type I error in these multiple comparisons, the Benjamini-Hochberg (BH)/false-discovery-rate (FDR) corrections to significance were applied to the significant P values where a single asterisk (*) represents 0.05 or less, two asterisks (**) represent 0.01 or less, three asterisks (***) represent 0.001 or less, and four asterisks (****) represent 0.0001 or less.