TABLE 5.
Comparison of RT mutation frequencies versus WT: class 3 multiple nucleotide substitution mutations (non-ADAR/non-APOBEC)d
| Enzyme ID | Mutation frequencya | Mutation rateb | Mutation fractionc (%) | Raw (FET) P value | BH (FDR)-adjusted P value | BH significance |
|---|---|---|---|---|---|---|
| WT | 6/205,171 | 1.9 × 10−7 | 1 | |||
| Y115F | 10/93,725 | 7.0 × 10−7 | 2 | 0.0125 | 0.0332 | * |
| Q151M | 5/209,064 | 1.6 × 10−7 | 1 | 0.7724 | 0.7724 | |
| M184I | 17/150,110 | 7.4 × 10−7 | 4 | 0.0026 | 0.0209 | * |
| M184V | 7/172,601 | 2.7 × 10−7 | 2 | 0.5885 | 0.6725 |
Mutation frequency data represent the number of mutant lacZα sequences divided by the total number of recovered clones.
Mutation rate data represent the mutation frequency divided by the size of the lacZα target sequence. Because the data were obtained with a one-round vector, the rate data were calculated as mutations/bp/generation.
Mutation fraction data represent the number of class-specific mutant lacZα sequences divided by the total number of mutant lacZα.
Raw (FET) data represent P values of the significance of the results of comparisons to the WT by Fisher's exact test. To control for compounding type I error in these multiple comparisons, the Benjamini-Hochberg (BH)/false-discovery-rate (FDR) corrections to significance were applied where a single asterisk (*) represents 0.05 or less, two asterisks (**) represent 0.01 or less, three asterisks (***) represent 0.001 or less, and four asterisks (****) represent 0.0001 or less.