FIG 3.

Differential regulation of secreted protein and surface membrane-bound immunomodulatory protein expression following cytokine exposure. At 8 days (effector) or >60 days (memory) after LCMV infection, T cells were pretreated with medium or IL-4, IL-10, and TGF-β (10 ng/ml each) for 2 h, followed by stimulation with IL-12 plus IL-18, IL-12 plus TNF-α, or IL-12 plus IL-15 (10 ng/ml) for 6 h. (A) Innate upregulation of CD25 expression and IFN-γ production by virus-specific CD8⁺ effector T cells. The numbers in each quadrant represent the percentage of NP118 tetramer⁺ CD8⁺ T cells expressing IFN-γ and/or CD25, while the numbers in parentheses represent the MFI of IFN-γ⁺ T cells. Data are representative of 4 mice from 2 independent experiments. (B and C) Surface marker upregulation and IFN-γ production in response to proinflammatory cytokine stimulation following pretreatment with medium only (solid symbols) or medium containing inhibitory cytokines (open symbols). Each data point represents the percentage of NP18-tetramer⁺ CD8⁺ T cells that expressed IFN-γ and CD25 (B) or IFN-γ and CD69 (C) following treatment with IL-12 plus IL-18 (circles), IL-12 plus TNF-α (squares), IL-12 plus IL-15 (triangles), or medium alone (diamonds). Data represent the average ± SD from 4 mice per group from 2 independent experiments.