FIG 1.

Extracellularly added E^rns inhibits Mx induction by poly(I·C) in nonbovine cells. Confluent GSM cells (A), LSM cells (B), canine keratinocytes (C), and HUVECs (D) were stimulated with poly(I·C) for 20 h in the presence or absence of E^rns or the RNase-inactive mutant E^rns H30F at various concentrations, as indicated. Cell culture medium (MEM) and E^rns applied at the highest concentrations used, but in the absence of poly(I·C), served as negative controls. Cytosolic extracts were assayed for Mx and β-actin expression by Western blotting. Typical results out of three independent experiments are shown.