FIG 2.
Erns remains active for at least 72 h. BT cells were incubated in the presence of wt Erns protein or the RNase-inactive mutant H30F for 1 h prior to washing the cells with MEM. Thereafter, cells were incubated for 3 days (72 h, as indicated above the lanes) or 6 days (144 h) in fresh medium. Samples incubated for 6 days were detached with trypsin-EDTA and passaged at a ratio of 1 to 2 in new flasks after 3 days. Subsequently, poly(I·C) stimulation was performed for 20 h. The samples were probed for Mx and β-actin protein expression by Western blotting as described in the legend to Fig. 1. A typical result out of three independent experiments is shown.