FIG 4.
Inhibitors of clathrin-dependent endocytosis interfere with Erns uptake. BT cells were preincubated in the presence of 25 μM CPZ or 0.2 mM MDC (A) and 10 mM MβCD or 20 μM EIPA (B) for 30 min. Thereafter, cells were supplemented with Erns and incubated in the presence of the inhibitors for another hour. Washing of the cells with soluble heparin or medium was performed prior to stimulation of the cells with poly(I·C) for 18 h, followed by analysis for Mx and β-actin expression by Western blotting. Typical results out of three independent experiments are shown.