FIG 4.

The expression of wild-type and mutant MAGI-1 promotes junctional assembly in HeLa cells. (A) HeLa cells were seeded on glass coverslips and transfected either with wild-type or mutant MAGI-1. After 24 h, the cells were fixed and incubated with anti-FLAG and anti-ZO-1 antibodies and counterstained with rhodamine-conjugated (FLAG) and fluorescein-conjugated (ZO-1) secondary antibodies. Confocal images were taken at 480- and 510-nm wavelengths. (B) Cells were seeded and transfected as described for panel A. After fixation, cells were incubated with anti-FLAG and anti-PAR3 antibodies and counterstained with rhodamine-conjugated (FLAG) and fluorescein-conjugated (PAR3) secondary antibodies. Confocal images were taken at 480- and 510-nm wavelengths. Arrows indicate sites of ZO-1 and PAR3 junctional accumulation.